The following links are for Laragen Sequencing Sample Preparation
1. Ready-To-Run 2. Premix 3. Purified Templates 4. Unpurified PCR products 5. RCA sequencing 6. Primer walking 7. Difficult Templates |
| Laragen In House Primer List |
| M13 Forward | GTA AAA CGA CGG CCA GT |
| M13 Reverse | CAG GAA ACA GCT ATG AC |
| T7 Promoter | TAA TAC GAC TCA CTA TAG GG |
| T3 | ATT AAC CCT CAC TAA AG |
| SP6 | ATT TAG GTG ACA CTA TAG |
| T7 Terminator | CTA GTT ATT GCT CAG CGG TG |
| pGEX 5' | GGG CTG GCA AGC CAC GTT TGG TG |
| pGEX 3' | CCG GGA GCT GCA TGT GTC AGA GG |
| RV3 | CTA GCA AAA TAG GCT GTC CCC |
| BGH Reverse | TAG AAG GCA CAG TCG AGG C |
| pBADfor | ATG CCA TAG CTT TTT ATC C |
| pBADrev | GAT TTA ATC TGT ATC AGG |
| pFastBacFwd | TAT TCC GGA TTA TTC ATA CCG TC |
| pFastBacRev | GTA TGG CTG ATT ATG ATC CTC |
| CMVfor | CGC AAA TGG GCG GTA GGC GTG |
| CMVRev | AGT AGG AAA GTC CCG TAA GG |